A Preliminary Approach to the Impact of a Commercial Formulation of Glyphosate (Roundup®) in Ecologically Relevant Concentrations on Pseudis minuta Tadpoles

Authors

  • Natália Medeiros de Albuquerque Wingen Programa de Pós-Graduação em Ecologia e Evolução da Biodiversidade, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brasil
  • Gustavo Kasper Cubas Curso de Ciências Biológicas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil
  • Guendalina Turcato Oliveira Programa de Pós-Graduação em Ecologia e Evolução da Biodiversidade, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brasil.

DOI:

https://doi.org/10.5132/eec.2022.01.07

Abstract

Freshwater natural environments are suffering with human impacts and economic activities. Agriculture is one of the most environmental impactful activities that uses several toxic agents to ecosystems, polluting and poisoning non-target species. The goal of this study was to evaluate the Brazilian most utilized herbicide impacts in larval fase of an exclusively aquatic anuran species. We collected Pseudis minuta eggs mass in a Butiazal reservation, inside a soy and rice farm. In lab, we exposed 25 hatched tadpoles to 65μg/L, 250μg/L and 500μg/L of glyphosate and we had a control group that was kept in clean water. After exposition (seven days), we analyzed growing and morphometric patterns, oxidative balance (Superoxide Dismutase, Catalase and lipoperoxidation levels) and biotransformation (Glutathione S-transferase) parameters. We have not observed significatively differences about morphometric parameters, however, tadpoles grew above the expected in concentration 65 μg/L. There were not significatively differences between any oxidative balance marker, suggesting a tolerance or resistance level of this species. We do not discard this possibility because glyphosate is the most used pesticide in Rio Grande do Sul and in Brazil. It is possible that Pseudis minuta, in this developmental stage (larval), doesn’t configure a good sentinel model organism. This work is a preliminary approach given the low number of tadpoles obtained from egg mass and consequently low number of replicates of exposure concentrations. Thus, future experiments should be designed to confirm these findings. The use of this type of assay may stimulate the development of new studies to assess the toxic risk of xenobiotics.

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Published

10-08-2022

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Original Articles