Impact of Exposure Duration on DNA Damage Scores in the Comet Assay Using Allium cepa L. and Lactuca sativa L.

Authors

  • Larissa Andrade-Vieira Department of Ecology and Conservation, Federal University of Lavras (UFLA), MG, Brazil
  • Franceli Aparecida da Cruz Department of Biology, Federal University of Lavras (UFLA), Lavras, MG, Brazil
  • Graciele Lurde Silveira Department of Biology, Federal University of Lavras (UFLA), Lavras, MG, Brazil
  • Silvana Marcussi Department of Chemistry, Federal University of Lavras (UFLA), Lavras, MG, Brazil

DOI:

https://doi.org/10.5132/eec.2024.01.04

Keywords:

Alkaline comet assay, Genotoxic, Methyl methanesulfonate, Mutagenic, Root tips, Single cell gel electrophoresis

Abstract

The single-cell gel electrophoresis (SCGE), or comet assay, is a simple method that uses microgel and electrophoresis to evaluate damage in the DNA of the cells. The first protocol adapted for plants was described in the 1990s, however, a universal standardized procedure is not available to date. This study aimed to evaluate the influence of exposure time in the comet assay in plant models Allium cepa L. and Lactuca sativa L. Root tips of both plant models were exposed to methyl methanesulfonate (MMS), a known genotoxicant, for 2 h to 36 h. The alkaline version of the comet assay for plant samples was performed, and the DNA damage was assessed. Arbitrary units (AU) were calculated and compared to the negative control. MMS induced DNA damage (P < 0.05) after 2, 6, 10, 14, 28, 32 and 36 h of exposure in A. cepa, and at 2, 8, 10, 14 and 36 h for L. sativa. The highest AU values for A. cepa were observed after 4 h of exposure to MMS and for L. sativa, after 24 h of exposure. The optimal exposure times considering both genotoxic and mutagenic risk were indicated.

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Published

23-07-2024

How to Cite

Andrade-Vieira, L., Cruz, F. A. da, Silveira, G. L., & Marcussi, S. (2024). Impact of Exposure Duration on DNA Damage Scores in the Comet Assay Using Allium cepa L. and Lactuca sativa L. Ecotoxicology and Environmental Contamination, 19(1), 30–38. https://doi.org/10.5132/eec.2024.01.04

Issue

Vol. 19 No. 1 (2024)

Section

Original Articles

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Copyright (c) 2024 Ecotoxicology and Environmental Contamination

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