Fishing activity in the Itajaí harbor (SC) has concentrated a large fishing fleet, operating with many different methods of capture. This paper presents a checklist of the species of elasmobranchs landed in Itajaí, and analyses the respective landing frequencies in the fishing methods. Elasmobranchs were observed in catches of purse seine, single trawl, double rig trawl, pair trawl, surface longline, bottom longline, surface gillnet, bottom gillnet and traps. In total, 85 species were found (54 sharks and 31 skates and rays) and of them, 22 shark species and 6 skates were very abundant in at least one fishing art.