This study presents the first report of infection by Linguadactyloides brinkmanni Thatcher & Krytsky, 1983 (Monogenoidea, Dactylogyridae, Linguadactyloidinae) on the gills of the hybrid tambatinga (Colossoma macropomum x Piaractus brachypomus) from fish farms from the city of Macapá, state of Amapá, eastern Amazonia, Brazil. Out of 201 hybrid tambatinga examined, ten had their gills infested by L. brinkmanni (prevalence= 4.9%). From these hosts, 21 specimens of L. brinkmanni were collected and the mean intensity of infestation was 2.1 (1-4 parasites). This study expands the distribution of this monogenoidean for a new host.