The genotoxic effects of metals were evaluated in Bathygobius soporator using the micronucleus test. The frequencies of micronuclei in fish erythrocytes cells of sampling points were elevated when compared to the controls (P < 0.05). Micronucleus test in fish erythrocytes were useful to determine genotoxic effects of metals substances in the fish, reducing the pain and suffer of the animal testing. The first PC (70.56%) of principal component analysis (PCA) account for almost all of the variance, and Pearson’s correlation coefficient between the first PC and micronucleus frequency of sampling points (P1-P10) was performed (r = -0.704, P = 0.023).