Tissue damage, mutagenic effect and alteration in antioxidant defense in Danio rerio (Cypriniformes: Cyprinidae) after chronic exposure to Metformin hydrochloride.

Authors

  • M.Sc. Natani Ribeiro Demarco Biosciences and Health Postgraduate Program, Universidade Estadual do Oeste do Paraná (UNIOESTE). Cascavel – PR, Brazil.
  • M.Sc. Bárbara Zanardini de Andrade Biosciences and Health Postgraduate Program, Universidade Estadual do Oeste do Paraná (UNIOESTE). Cascavel – PR, Brazil.
  • Matheus Felipe Zazula Molecular and Cell Biology PostGraduate Program, Universidade Federal do Paraná. Avenida Coronel Francisco H. dos Santos, 100 – 81531-980. Curitiba – PR, Brazil.
  • M.Sc. Thaís Maylin Sobjak Cell Biology Postgraduate Program, Universidade Federal de Minas Gerais (UFMG). Av. Antônio Carlos, 6627, Pampulha - Belo Horizonte - MG - CEP 31270-901, Brazil.
  • Dr. Luciana Paula Grégio D’Arce Craniofacial Anomalies Research and Care Center (CEAPAC), Universidade Estadual do Oeste do Paraná. Avenida Tancredo Neves, 3224 - 85806-470. Cascavel – PR, Brazil.
  • Ana Tereza Bittencourt Guimarães Universidade Estadual do Oeste do Paraná

DOI:

https://doi.org/10.5132/eec.2022.02.12

Keywords:

Environmental contamination, Mutagenicity, Micronucleus, Oxidative stress, Xenobiotics, Zebrafish

Abstract

Metformin hydrochloride (MET) is an oral medication prescribed for patients with diabetes, with a large part of its metabolites being eliminated in the urine. Basic sanitation systems are not fully effective in removing all the contaminants, promoting contamination to rivers and supply reservoirs. The objective of this study was to test the potential effects of MET on the antioxidant system, mutagenic and tissue damages in D. rerio. The animals were acclimatized and separated into six groups and exposed to different concentrations of MET (0.00, 0.25, 0.50, 0.75, 1.25 g.L-1) over a 96 hour period to determine the LC50. In another experiment, five groups of ten animals were separated: four groups for evaluation of the chronic effect of 0.45 g.L-1 of metformin hydrochloride (15, 30, 45 and 60 days) and, a negative control group (NC). The antioxidant system and the tissue damages of the muscle samples, the mutagenicity in the blood samples. The presence of oxidative stress and mutagenicity, together with the activation of the antioxidant system, showed up to 30 days of treatment, with intense tissue damages in 60 days and the emergence of apoptotic cells, which is evidence of the toxic potential of this drug to non-target aquatic populations.

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Published

08-05-2023

How to Cite

Ribeiro Demarco, N., Zanardini de Andrade, B., Zazula, M. F., Sobjak, T. M., Grégio D’Arce, L. P., & Guimarães, A. (2023). Tissue damage, mutagenic effect and alteration in antioxidant defense in Danio rerio (Cypriniformes: Cyprinidae) after chronic exposure to Metformin hydrochloride. Ecotoxicology and Environmental Contamination, 17(2), 115–125. https://doi.org/10.5132/eec.2022.02.12

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Original Articles

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