Micronucleus test for monitoring the genotoxic potential of the surface water of Luján River (Argentina) using erythrocytes of Lithobates catesbeianus tadpoles
Abstract
Luján river is a peri-urban ecosystem dominated by urban, industrial and agricultural discharges, receiving contributions from about one hundred streams along its path. The toxicological quality of the surface water in the middle course of the river was monitored by means of the micronucleus (MN) test, using peripheral erythrocytes of Lithobates catesbeianus premetamorphic tadpoles. This cytogenetic assay was performed to evaluate the effects of a particular domestic/industrial effluent poured continuously into the river, by incubating the larvae for 6 days to water samples from two sites: upstream and downstream the point where the effluent is dumped. The positive control was a cyclophosphamide solution. Five seasonal samplings were conducted between 2007 and 2008. The physicochemical analyses and the values of three water quality indices showed a highly polluted condition of the river. The MN frequency in the erythrocytes of the larvae exposed to the river samples was significantly higher than that of negative controls in all the cases and, in most samples, without differences with respect to the positive controls or even higher. There were no significant differences in the frequency of MN between the two environmental samples. The results allowed showing the genotoxic potential of the Lujan river water.
Downloads
Downloads
Published
How to Cite
Issue
Section
License
Copyright © 2006 ECOTOX-Brasil
Copyright notice: It is a condition for publication that manuscripts submitted to this journal have not yet been published and will not be simultaneously submitted or published elsewhere. By submitting a manuscript, the authors agree that copyright for their article is transferred to the Sociedade Brasileira de Ecotoxicologia (ECOTOX-Brasil) if and when the article is accepted for publication. The copyright covers the exclusive rights to reproduce and distribute articles, including reprints, photographic reproductions or any other reproduction of a similar nature, including translations. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without permission of the publisher.
Notice: While every effort is made by the EEC, editors and editorial board to see that no inaccurate or misleading data, opinions or statements appear in this journal, they wish to make it clear that the contents of the articles and advertisements published herein are the sole responsibility of the contributors or advertisers concerned. Accordingly, the EEC, the editorial board and editors and their respective employees, officers and agents accept no responsibility or liability whatsoever for the consequences of any inaccurate or misleading data, opinion or statement.
