Piscine micronucleus test and the comet assay reveal genotoxic effects of Atrazine herbicide in the neotropical fish Rhamdia quelen
DOI:
https://doi.org/10.5132/eec.2015.01.09Resumo
Atrazine (ATZ) is an herbicide extensively used around the world to kill weeds. Due to its applicability and benefits in farming, ATZ can easily reach the aquatic ecosystems and, therefore, represent risks for aquatic biota and human populations. The aim of this study was to evaluate the genotoxic effects of ATZ on Rhamdia quelen through the piscine micronucleus test (MNT) and the comet assay in erythrocytes (ECA) and gill cells (GCA), at three different concentrations (2, 10, and 100 μg L-1) in static (SB) and semi-static bioassays (SSB) during 96 hours. In the SB, we observed an increased frequency of nuclear morphological abnormalities at all concentrations and a dose-dependent effect of ATZ on DNA through the ECA. There was no difference among treatments in gills. In the SSB there was no significant difference in MNT, but the ECA showed an increase of DNA breakages at 10 μg L-1 treatment. GCA showed higher DNA damage on fish exposed to 2 and 100 μg L-1. Our results show a trend to dose-dependent genotoxic effect of ATZ, which causes damage to the DNA of Rhamdia quelen even in a concentration considered safe by regulatory agencies.Downloads
Downloads
Publicado
Como Citar
Edição
Seção
Licença
Copyright © 2006 ECOTOX-BRAZIL
Copyright notice: It is a condition for publication that manuscripts submitted to this journal have not yet been published and will not be simultaneously submitted or published of elsewhere. By submitting a manuscript, the authors agree that copyright for their article is transferred to the Sociedade Brasileira de Ecotoxicologia (ECOTOX-Brazil) if and when the article is accepted for publication. The copyright covers the exclusive rights to reproduce and distribute articles, including reprints, photographic reproductions or any other reproduction of a similar nature, including translations. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without permission of the publisher.
Notice: While every effort is made by the JBSE, editors and editorial board to see that no inaccurate or misleading data, opinions or statements appear in this journal, they wish to make it clear that the contents of the articles and advertisements published herein are the sole responsibility of the contributors or advertisers concerned. Accordingly, the JBSE, the editorial board and editors and their respective employees, officers and agents accept no responsibility or liability whatsoever for the consequences of any inaccurate or misleading data, opinion or statement.
