A acidificação dos oceanos resulta da absorção de cerca de um terço das emissões de CO2 atmosférico levando a uma diminuição do pH da água do mar. Este fenómeno pode causar danos subletais ou letais aos organismos marinhos, alguns dos quais são cruciais para os ecossistemas costeiros e para a economia humana. Reproduzir a água do mar acidificada em laboratório é essencial para avaliar potenciais impactos sobre esses organismos em ensaios de tolerância. O protocolo executado utilizou uma atmosfera enriquecida com CO2 para determinar a tolerância ao pH de Mysidopsis juniae em ensaios letais e de larvas de Arbacia lixula em ensaios crônicos. M. juniae apresentou respostas letais em pH 6,8 ± 0,3, enquanto A. lixula exibiu atraso no desenvolvimento larval como reação subletal em pH 7,2 ± 0,3. No entanto, estes níveis de pH são extremos e não podem ser previstos para futuras condições oceânicas. Outros estudos indicaram efeitos subletais negativos em organismos semelhantes em níveis de pH mais elevados do que os avaliados neste estudo. Assim, são necessários outros estudos com parâmetros fisiológicos mais adequandos para uma correta e segura avaliação dos perigos da acidificação dos oceanos podem ocasionar para os organismos marinhos.
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